Antibacterial activity of medicinal plants on the management of mastitis in dairy cows: A systematic review.

BACKGROUND: Mastitis is a disease of economic importance in dairy production systems. The common management regime for mastitis is the use of synthetic antibiotics, giving a new problem of antibiotic resistance. There is, therefore, a need to prospect for alternatives to conventional antibiotics from herbal plants. OBJECTIVES: This systematic review evaluates the use of plants as alternatives for the control of mastitis in dairy cattle, focussing on the effectiveness of studied plants and plant-based products and possible implications on the use of these products in livestock health. METHODOLOGY: The PRISMA model was implemented with searches done in five electronic databases: Scopus, ScienceDirect, PubMed, Ovid and Research4Life. Data were extracted from 45 studies with 112 plant species from plant species belonging to 42 different families. The specific keywords were 'mastitis', 'dairy cows' and 'medicinal plants'. RESULTS: The most cited plant species included Allium sativum L., Azadirachta indica and Eucalyptus globulus Labill with the latter further exploring its components. Microbial species causing mastitis mainly were Staphylococcus aureus and Escherichia coli. The extraction methods used included maceration approach using ethanol, methanol and water as solvents for phytochemicals and chromatographic techniques for essential oils. A few studies explored the mode of action, and toxicities of the herbal extracts as well as evaluating their efficacy in clinical trials using animal models. CONCLUSION: Plants with defined levels of phytochemicals were essential sources of antibacterials. Standardisation of analytical methods is required.

Enteric methane emission estimates for the Zimbabwean Sanga cattle breeds of Tuli and Mashona.

The effectiveness of methane mitigation in ruminant livestock production systems depends on the accuracy of estimating methane emission factors and providing accurate emission inventories. Following the Paris Climate agreement, it is recommended that countries adopt the Tier-2 approach for estimating enteric methane emissions from ruminants instead of the Tier-1 approach currently used by most countries. This study sought to provide base line enteric methane emission estimates for the Tuli and Mashona Sanga cattle breeds in Zimbabwe using the IPCC Tier-2 model. Using animal characterization data collected from 412 cattle from Grasslands Research Institute and 406 cattle from Makoholi Research Institute, net energy requirements were estimated. From this and the estimate for digestibility, gross energy intake and dry matter intake were estimated. Gross energy intakes and the estimated methane conversion factor were used to estimate enteric methane emissions. Mean emission factors for Tuli were 45.1, 56, 28.5, 28.4 and 20.6 kg CH4/head/year for cows, bulls, heifers, steers and calves, respectively. For Mashona, they were 47.8, 51.9, 29, 29.1 and 20.7 kgCH4/head/year for cows, bulls, heifers, steers and calves, respectively. Generally, estimated Tier-2 emission factors were significantly different from the IPCC Tier-1 default emission factors. This study concluded that enteric methane emission factors estimated using the IPCC Tier-2 model offer insights into the controversial use of the default IPCC Tier-1 emission factors.

Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe.

BACKGROUND: Local communities in the South Eastern Lowveld of Zimbabwe have adopted the feeding of livestock with Neorautanenia brachypus (Harms) C.A. tuber to mitigate against climate change. Differences within Neorautanenia brachypus (Harms) tuber flesh colour and preferences by cattle have been observed, suggesting possible diversity within the N. brachypus plant community. This study aimed at distinguishing the N. brachypus wild plant species through phenotypic and genetic characterization using morphological descriptors and random amplified polymorphic (RAPD) markers respectively. Leaf samples were selected using judgmental sampling techniques from wards 11-15 in Sengwe (Chiredzi district) for leaf morphology and molecular characterization. RAPD-PCR analysis was done using 18-screened random decamer primers to confirm the diversity in the plant population. The similarity of the biotypes was evaluated using binary coding on the basis of the presence or absence of a morphological indicator as well as distinct DNA amplicon fragments. Primer 7.0.13 was used to estimate morphological and genetic similarities using the unweighted pair group method with arithmetic average (UPGMA). The cluster number was estimated using the Elbow method part of the R package. RESULTS: Initially, 14 biotype groups were identified from 96 accessions visually characterized basing of leaf characteristics. All the leaf biotypes displayed arcuate venation with differences observed for leaf shape, tip shape and leaf margins. The 14 biotypes clustered into six groups based on the binary data of the morphological characteristics. RAPD primers generated three hundred and sixty eight distinct amplicons with 77.5% being polymorphic from the 14 biotypes. The number of bands produced per primer ranged from four (OPF-02) to 44 (UBC-746). The PIC value ranged from 0.1327 to 0.1873 for the RAPD primers. Use of molecular markers collapsed the biotypes into five clusters. Both the leaf descriptors and RAPD markers showed the existence of genetic diversity within the wild accessions of N. brachypus. CONCLUSIONS: A combination of morphological and RAPD markers effectively refined the resolution of the genetic diversity within the N. brachypus wild accessions to nine biotypes. These findings have indicated to the existence of more than one biotype of N. brachypus with potentially different properties. The favorable biotypes can further be promoted through incorporation in pastures as alternative feed or complementary feed to livestock. As such the output of this study will serve as a guide for N. brachypus germplasm management and improvement.

Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management.

CONTEXT: Salvadora persica L. (Salvadoraceae), Colophospermum mopane (J.Kirk ex Benth.) J. Léonard (Leguminosae) and Dichrostachys cinerea (L.) Wight & Arn. (Leguminosae) crude extracts are used by local farmers against many livestock infections with little or no side effects usually associated with synthetic antimicrobials. However, their efficacy has rarely been tested. OBJECTIVE: These plants were tested for potential antibacterial activity against clinical isolates of Staphylococcus aureus ATCC33862 and Escherichia coli ATCC25922. Minimal inhibitory concentrations (MIC) of the crude plant extracts were determined. MATERIALS AND METHODS: Aqueous and methanol extraction of 100 g each of the bark of C. mopane, roots of D. cinerea and leaves of S. persica was done by placing the samples in 250 mL of either water or methanol. Nutrient broth was used as growth medium for the bacteria, and McFarland standard for bacterial standardization. 2,3,5-Triphenyltetrazoliumchloride (TTC) was the indicator salt. Each of the aqueous and methanol extracts (100 µL) was tested. Gentamycin and ampicillin were the controls. RESULTS: MIC of aqueous extracts ranged from 1.03-14.6 mg/mL against S. aureus, and from 12.1-34.3 mg/mL against E. coli. Methanol extracts ranged between 5.31 and 9.64 mg/mL against S. aureus, and between 7.86 and 13.6 mg/mL against E. coli. Aqueous and methanol extracts of S. persica were significantly higher (p < 0.05) than C. mopane and D. cinerea. DISCUSSION AND CONCLUSION: Colophospermum mopane, S. persica and D. cinerea exhibited antibacterial activity, with methanol extracts performing better than aqueous extracts, justifying use as ethnoveterinary medicine. Further study to isolate the active components should be pursued.